FAK [pY407] Antibody | catalog# XBP-4113
Background
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that was discovered as a substrate for Src, and is a key element of integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. Phosphorylation of tyrosine 407 is activated by TGF? and Epithelial Mesenchyme Transition (EMT). Unlike other sites on FAK, tyrosine 407 is EGFR independent.
Description
![FAK [pY407] Antibody](./image/product/08/19/XBP-4113.gif)
Extracts of primary chick embryo fibroblasts plated on fibronectin (1) and expressing human FAK (2-5) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with the FAK [pY407] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the phosphopeptide immunogen (3), a generic phosphotyrosine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F(ab')2 anti-rabbit IgG-HRP conjugate, and signals were detected using the Pierce SuperSignal™ method.
Specifications
Formulation
Rabbit polyclonal immunoglobulin in Dulbecco's phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG, protease free) as a carrier.Clonality/Clone
This is a polyclonal antibody.Specificity
Human FAK. Mouse, rat, chicken (100% homologous) and frog (92% homologous) FAK have not been tested, but are expected to react.Source
FAK antibody was produced against a chemically synthesized phosphopeptide derived from the region of human FAK that contains tyrosine 407. The sequence is conserved in mouse, rat and chicken.Purification
FAK antibody was purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using (1) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated FAK and (2) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using a FAK-derived peptide that is phosphorylated at tyrosine 407.Host
FAK [pY407] antibody was raised in rabbit.Please use anti-rabbit secondary antibodies.
Application
For Western blotting applications, we recommend using the antibody at a 1:1000 starting dilution.Tested Application
WBBuffer
Rabbit polyclonal immunoglobulin in Dulbecco's phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG, protease free) as a carrier.Storage
Store at -20˚C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20˚C. For short-term storage (up to one week), 2 - 8˚C is sufficient.Positive Control
- Chicken embryo fibroblast (CEF) cells expressing FAK protein and plated on fibronectin.]
Species Reactivity
H, M, R, C, XReference
- Lu, H., et al. (2006) The microtubule binding drug laulimalide inhibits VEGF-induced human endothelial cell migration, and is synergistic when combined with Taxotere (docetaxel). Mol. Pharmacol. [Epub ahead of print].
- Eliceiri, B.P., et al. (2002) Src-mediated coupling of focal adhesion kinase to integrin alpha(v)beta5 in vascular endothelial growth factor signaling. J. Cell. Biol. 157(1):149-160.
- Lim, Y., et al. (2002) Trichostatin A-induced detransformation correlates with decreased focal adhesion kinase phosphorylation at tyrosine 861 in ras-transformed fibroblasts. J. Biol. Chem. 277(15):12735-12740.
- Nakamura, K., et al. (2001) Different modes and qualities of tyrosine phosphorylation of Fak and Pyk2 during epithelial-mesenchymal transdifferentiation and cell migration: analysis of specific phosphorylation events using site-directed antibodies. Oncogene 20(21):2626-2635.]