FAK [pS910] Antibody | catalog# XBP-4110
Background
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that was discovered as a substrate for Src, and is a key element of integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. Tyrosine phosphorylation of FAK is associated with positive regulation and growth promotion, while serine phosphorylation is associated with negative regulation, often during mitosis or cell adhesion.
Description
![FAK [pS910] Antibody](./image/product/17/08/XBP-4110.gif)
Extracts of human epithelial carcinoma cells expressing FAK with (2-5) or without (1) 100 ng/mL Taxol for 18-24 hours in serum-free media to arrest cells in mitosis were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST for one hour at room temperature, then incubated with the FAK [pS910] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1,2), the non-phosphorylated peptide corresponding to the phosphopeptide immunogen (3), a generic phosphoserine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F(ab')2 anti-rabbit IgG HRP-conjugate and the signals were detected using the Pierce SuperSignal™ method.
Specifications
Formulation
Rabbit polyclonal immunoglobulin in Dulbecco's phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG, protease free) as a carrier.Clonality/Clone
This is a polyclonal antibody.Specificity
Human FAK. Mouse, rat, chicken and frog (100% homologous) FAK have not been tested, but are expected to react.Source
FAK antibody was produced against a chemically synthesized phosphopeptide derived from the region of human FAK that contains serine 910. The sequence is conserved among multiple species including mouse, rat, chicken and frog.Purification
FAK antibody was purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated FAK enzyme and (ii) a generic serine phosphorylated peptide to remove antibody that is reactive with phospho-serine, irrespective of the sequence. The final product is generated by affinity chromatography using a FAK-derived peptide that is phosphorylated at serine 910.Host
FAK [pS910] antibody was raised in rabbit.Please use anti-rabbit secondary antibodies.
Application
For Western blotting applications, we recommend using the antibody at a 1:1000 starting dilution. The optimal antibody concentration should be determined emperically for each specific applicationTested Application
WBBuffer
Rabbit polyclonal immunoglobulin in Dulbecco's phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG, protease free) as a carrier.Storage
Store at -20˚C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20˚C. For short-term storage (up to one week), 2 - 8˚C is sufficient.Positive Control
- Human epithelial carcinoma cells arrested in mitosis by treatment with Taxol.]
Species Reactivity
H, M, R, C, XReference
- Ma, A., et al. (2001) Serine phosphorylation of focal adhesion kinase in interphase and mitosis: A possible role in modulating binding to p130Cas. Mol. Biol. Cell 12(1):1-12.
- Xu, L.H., et al. (2000) The focal adhesion kinase suppresses transformation-associated, anchorage-independent apoptosis in human breast cancer cells: involvement of death receptor-related signaling pathways. J. Biol. Chem. 275(39):30597-30604.
- Yamakita, Y., et al. (1999) Dissociation of FAK / p130(CAS) / c-Src complex during mitosis: role of mitosis-specific serine phosphorylation of FAK. J. Cell Biol. 144(2):315-324.
- Richardson, A., et al. (1997) Identification of integrin-stimulated sites of serine phosphorylation in FRNK, the separately expressed C-terminal domain of focal adhesion kinase: a potential role for protein kinase A. Biochem. J. 324 (Pt 1):141-149.]