FAK [pS732] Antibody | catalog# XBP-4108

Background

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that plays a key role in signaling by growth factors, extracellular matrix and stress signals. Indeed, FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. The serine/threonine kinase cyclin-dependent kinase-5 (Cdk5) - an important regulator in brain development - phosphorylates serine 732 of FAK, possibly regulating a centrosome-associated microtubule structure to promote nuclear translocation, a critical step in neuronal migration. This phosphorylation does not have a direct impact on the kinase activity of FAK, but appears to prevent the accumulation of FAK at the centrosome.

Description

Extracts of PC12 cells treated with 100 ng/mL nocozadole for 2 hours were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with the FAK [pS732] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the phosphopeptide immunogen (2), a generic phosphoserine-containing peptide (3), or the phosphopeptide immunogen (4). After washing, the membrane was incubated with goat F(ab')2 anti-rabbit IgG HRP conjugate and signals were detected using the Pierce SuperSignal™ method.