ProSci Incorporated

Antibodies For All Your Research

Production and Services

FAK Antibody | catalog# XBP-4011

Background

This antibody recognizes a protein of Mr=125 kDa, identified as focal adhesion kinase (FAK). FAK is a widely expressed non-receptor protein tyrosine kinase discovered as substrate for Src and as a key element of integrin signaling. Regulation of FAK include phosphorylation at multiple tyrosine and serine residues. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion; however, dephosphorylation at these sites occurs as cells begin to enter the M-phase of the cell cycle. In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK binds multiple signaling proteins including p130Cas, Graf, Grb2, Src family SH2 domains, and the p85 subunit of PI3-kinase.

SIZE
PRICE(Select options)QTY
Credit Card Processing

Specifications

Clonality/Clone
This is a monoclonal antibody.
Specificity
Human, mouse and rat.
Formulation
Purified immunoglobulin in phosphate buffered saline, pH 7.2, with 1% bovine serum albumin.
Source
FAK antibody was produced against recombinant fragment of human FAK expressed in E. coli.
Host
FAK antibody was raised in mouse.

Please use anti-mouse secondary antibodies.
Application
FAK antibody is suitable for use in Western blotting.
Tested Application
WB
Buffer
Purified immunoglobulin in phosphate buffered saline, pH 7.2, with 1% bovine serum albumin.
Storage
Store at 2 - 8˚C. For long term storage, aliquot into small volumes and store at –20˚C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Positive Control
  • Human HeLa cells, mouse L929 cells and rat PC12 cells. ]
Species Reactivity
H
Reference
  1. Ilic, D., et al. (2004) FAK promotes organization of fibronectin matrix and fibrillar adhesions. J. Cell Sci. 117:177-187.
  2. Cao, Y., et al. (2005) Growth factors stimulate kidney proximal tubule cell migration independent of augmented tyrosine phosphorylation of focal adhesion kinase. Biochem. Biophys. Res. Com. 328:560-566.
  3. Dahmani, S., et al. (2004) Lidocaine increases phosphorylation of focal adhesion kinase in rat hippocampal slices. Eur. J. Pharmacol. 489:55-58.
  4. Dunty, J., et al. (2004) FERM domain interaction promotes FAK signaling. Mol. Cell. Biol. 24(12):5353-5368.]