EPAC1 Antibody | catalog# 6631
Background
EPAC1, also known as Rap guanine nuclear exchange factor 3 and cAMPGEF-I, is widely expressed but most prominently in brain, heart, kidney, pancreas, spleen, ovary, thyroid and spinal cord. EPAC1 is a cAMP-binding protein with intrinsic guanine nuclear exchange factor activity that couples cAMP production to the activation of Rap, a GTPase belonging to the Ras family. This activation of Rap influences numerous cellular processes such as integrin-mediated cell adhesion, vascular endothelial barrier formation, and cardiac myocyte gap junction formation. Recently, EPAC1 has been suggested to also be involved in the cAMP-dependent regulation of ion channel formation, intracellular Ca++ signalling, ion transporter activity, and exocytosis.
Description
Western blot analysis of EPAC1 in rat skeletal muscle tissue lysate with EPAC1 antibody at 1 μg/mL.
Specifications
Protein Accession Number
NP_659099Purification
Affinity chromatography purified via peptide columnClonality/Clone
This is a polyclonal antibody.Host
EPAC1 antibody was raised in rabbit.Please use anti-rabbit secondary antibodies.
Specificity
At least two isoforms of EPAC1 are known to exist; this antibody will detect both isoforms. EPAC1 antibody is predicted to not cross-react with EPAC2.Immunogen
EPAC1 antibody was raised against an 18 amino acid peptide near the amino terminus of human EPAC1.Application
EPAC1 antibody can be used for detection of EPAC1 by Western blot at 1 - 2 μg/mL.Tested Application
E, WBBuffer
Antibody is supplied in PBS containing 0.02% sodium azide.Blocking Peptide
Cat. No. - 6631P - EPAC1 PeptideStorage
EPAC1 antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.Reference
- de Rooij J, Zwartkruis FJ, Verheijen MH, et al. Epac is a Rap1 guanine-nucleotide-exchange factor directly activated by cyclic AMP. Nature 1998; 396:474-7.
- Holz GG, Kang G, Harbeck M, et al. Cell physiology of cAMP sensor Epac. J. Physiol. 2006; 577:5-15.]
Positive Control
- Cat. No. 1467 - Rat Skeletal Muscle Tissue Lysate]