Clonality/CloneThis is a polyclonal antibody.
HostDARPP 32 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
ImmunogenRabbit anti-DARPP-32 (Thr34) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Thr34 of DARPP-32. Rabbit anti-DARPP-32 (Thr34) was purified by sequential chromatography on phospho-and non-phosphopeptide affinity columns.
ApplicationDARPP 32 antibody is specific for the ~32k DARPP-32 phosphorylated at Thr34. Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. Rabbit anti-DARPP-32 (Thr34) recognizes human, mouse, and rat forms of the protein. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
BufferDARPP 32 antibody is supplied as affinity purified polyclonal antibody, 100 μl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/ml BSA and 50% glycerol, and there is adequate amount of material to conduct 10-mini Western Blots.
StorageFor long term storage -80˚C is recommended, but shorter term storage at -20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20˚C.
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- Lindskog, M., Svenningsson, P., Pozzi, L., Kim, Y., Fienberg, A.A., Bibb, J.A.., Fredholm, B.B., Nairn, A.C., Greengard, P. and Fisone, G., “Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine, Nature (London), 418 (2002) 774 - 778.
- Maldve, R.E., Zhang, T.A., Ferrani-Kile, K., Schreiber, S.S., Lippmann, M.J., Snyder, G.L., Feinberg, A.A., Leslie, S.W., Gonzales, R.A., and Morrisett, R.A., “DARPP-32 and the regulation of the ethanol sensitivity of NMDA receptors in the nucleus accumbens,” Nature Neurosci. 5 (2002) 641 - 648.
- Bibb, J.A., Snyder, G.L., Nishi, A., Yan, Z., Meijer, L., Fienberg, A.A., Tsai, L.H., Kwon, Y.T., Girault, J.A., Czernik, A.J., Huganir, R.L., Hemmings, Jr., H.C., Nairn, A.C., and Greengard, P., “Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signalling in neurons, Nature (London) 402 (1999) 669 - 671.]