Cerebral Cortex Lysate

Cerebral Cortex Lysate | catalog# 21-277

Background

Monkey (Rhesus) cerebral cortex tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70˚C. The monkey (Rhesus) cerebral cortex tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the cerebral cortex tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The cerebral cortex tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

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Specifications

User Note

Ships on Dry Ice - Can only ship to U.S. Addresses

Disclaimer

This product is for research use only.

Tissue Type

Brain: Cerebral cortex

Diagnosis

Normal

Source

Monkey (Rhesus) brain: cerebral cortex tissue

Application

Cerebral cortex lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.

Tested Application

Storage

Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -70˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.