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Cerebellar Peduncles Lysate | catalog# XBL-10133

Background

Human cerebellar peduncles tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70˚C. The human cerebellar peduncles tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the cerebellar peduncles tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The cerebellar peduncles tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

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Specifications

User Note
Ships on Dry Ice
Disclaimer
This product is for research use only.
Tissue Type
Brain: Cerebellar Peduncles
Diagnosis
Normal
Source
Human Cerebellar Peduncles tissue
Application
Cerebellar Peduncles lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.
Tested Application
WB
Storage
Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -70˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
Species Reactivity
n/a
Species
Human