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CARMA1 Antibody | catalog# 3189

Background

CARMA1 Antibody: CARMA proteins belong to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARMA1, CARMA2, and CARMA3 share high degrees of sequence and functional homology, but their tissue-specific distribution suggests that they serve distinct biological functions in different cell types. Both CARMA1 and CARMA3 associate with NEMO, the regulatory subunit of the IκK complex, thereby regulating activation of the NF-κB transcription factor. Also, gene inactivation studies showed a complete block in T and B cell immunity as well as an impaired response to LPS, indicating that CARMA1 is a critical regulator in both the adaptive and innate immune systems.

Description
CARMA1 Antibody
Western blot analysis of CARMA1 expression in mouse thymus cell lysate with CARMA1 antibody at 0.5 (lane A), 1 (lane B), and 2 μg /ml (lane C), respectively.
CARMA1 Antibody
Immunohistochemistry of CARMA1 in mouse thymus with CARMA1 antibody at 10 μg/mL.
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Specifications

Additional Names
CARMA1
Additional Names
CARMA1 Antibody:
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Molecular Weight
125 kDa
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid

Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal CARMA1 Antibody.
Purification
CARMA1 Antibody is affinity chromatography purified via peptide column.
Host
CARMA1 antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.
Immunogen
CARMA1 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human CARMA1.
Application
CARMA1 antibody can be used for detection of CARMA1 by Western blot at 0.5 to 2 μg/mL. A band at approximately 125 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Tested Application
E, WB, IHC, IF
Buffer
CARMA1 Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3189P - CARMA1 Peptide
Storage
CARMA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
  • Cat. No. 1409 - Mouse Thymus Tissue Lysate]
Species Reactivity
H, M
Protein GI Number
172046231
Protein Accession Number
Q9BXL7
Reference
  1. Fanning AS and Anderson JM. Protein modules as organizers of membrane structure.Curr. Opin. Cell Biol. 1999; 11:432-9.
  2. Gaide O, Martinon F, Michau O, et al. Carma1, 1 CARD-containing binding partner of Bcl10, induces Bcl10 phosphorylation and NF-κ B activation. FEBS Lett. 2001; 496:121-7.
  3. Bertin J, Wang L, Guo Y, et al. CARD11 and CARD14 are novel caspase recruitment domain (CARD)/membrane-associated guanylate kinase (MAGUK) family members that interact with BCL10 and activate NF-κ B. J. Biol. Chem. 2001; 276:11877-82.
  4. McAllister-Lucas LM, Inohara N, Lucas PC, et al. Bimp1, a MAGUK family member linking protein kinase C activation to Bcl10-mediated NF-κB induction. J. Biol. Chem. 2001; 276:30589-97.]