CAD Antibody | catalog# 2011
Background
CAD Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40. Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.
Description
Specifications
Additional Names
CAD Antibody: DFF40Molecular Weight
40 kDaDisclaimer
This product is for research use only.User Note
Optimal dilutions for each application to be determined by the researcher.Formulation
Physical State: LiquidConcentration: 1 mg/mL
Isotype
IgGClonality/Clone
This is a polyclonal CAD Antibody.Conjugate
NonePurification
CAD Antibody is affinity chromatography purified via peptide column.Host
CAD antibody was raised in rabbit.Please use anti-rabbit secondary antibodies.
Immunogen
CAD antibody was raised against a peptide corresponding to amino acids 314 to 329 of murine CAD.Application
CAD antibody antibody can be used for detection of CAD by Western blot at 0.5 μg/mL. A 40 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 10 μg/mL.Tested Application
E, WB, IHC, IFBuffer
CAD Antibody is supplied in PBS containing 0.02% sodium azide.Blocking Peptide
Cat. No. 2011P - CAD PeptideStorage
CAD antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.Positive Control
- Cat. No. 1402 - Mouse Lung Tissue Lysate
- Cat. No. 1405 - Mouse Kidney Tissue Lysate]
Species Reactivity
MProtein GI Number
20137695Protein Accession Number
O54788Reference
- Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50
- Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99
- Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci USA 1998;95:8461-6
- Halenbeck R, MacDonald H, Roulston A, Chen TT, Conroy L, Williams LT. CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45. Curr Biol 1998;8:537-40 ]