Biotin Conjugated Affinity Purified anti-Mouse IgG [H&L] [Goat] Minimum Cross Reactivity to Human Serum Proteins

Biotin Conjugated Affinity Purified anti-Mouse IgG [H&L] [Goat] Minimum Cross Reactivity to Human Serum Proteins | catalog# XR-9740

Background

Specifications

User Note

USDA Certification: Products of animal origin are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation.

During shipment, small volumes of Biotin Conjugated Affinity Purified anti-Mouse IgG will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.

Conjugate

Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)

Purification

Biotin Conjugated Affinity Purified anti-Mouse IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Human Serum Proteins. Specificity was confirmed by ELISA at less than 1.0% cross reactivity against human IgG.

Host

Goat

Immunogen

Mouse IgG, whole molecule

Preparation

PHYSICAL STATE: Lyophilized

ANTIBODY CONCENTRATION: 2.0 mg/ml (by UV absorbance at 280 nm)

Application

Biotin Conjugated Affinity Purified anti-Mouse IgG is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assay using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:50,000 to 1:200,000 of the reconstitution concentration is suggested for this product. (Optimal dilution should be determined by user).

Tested Application

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Storage

Biotin Conjugated Affinity Purified anti-Mouse IgG can be stored vial at 4˚C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage mix product with glycerol to 50% and then aliquot contents and freeze at -20˚C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4˚C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

Species Reactivity

Formulation

STABILIZER:10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free

PRESERVATIVE: 0.01% (w/v) Sodium Azide

Reference

Bayer & Wilchek Methods in Enzymology 184; 138-160, 1990. ]