Beta-actin Antibody | catalog# 3779
Background
Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Description
Western blot analysis of beta-actin in HeLa cell lysate with beta-actin antibody at (A) 0.5, (B) 1 and (C) 2 μg/ml.
Immunocytochemistry of Actin in HeLa cells with Actin antibody at 1 μg/ml.
Specifications
Additional Names
Beta actin, b actinDisclaimer
This product is for research use only.Formulation
Physical State: LiquidConcentration: 1 mg/mL
Clonality/Clone
This is a polyclonal antibody.Purification
Affinity chromatography purified via peptide columnHost
Beta-actin antibody was raised in rabbit.Please use anti-rabbit secondary antibodies.
Immunogen
b-actin antibody was raised against a 16 amino acid peptide from near the amino-terminus of human b-actin.Application
b-actin antibody can be used for the detection of b-actin by Western blot at 1 - 2 μg/mL.Tested Application
E, WB, IHCBuffer
Antibody is supplied in PBS containing 0.02% sodium azide.Blocking Peptide
Cat. No. 3779P - B-actin PeptideStorage
Beta-actin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.Positive Control
- Cat. No. 1201 - HeLa Cell Lysate]
Species Reactivity
H, M, RProtein GI Number
12803203Protein Accession Number
AAH02409Reference
- Lambrechts A, Van Troys, M and Ampe C. The actin cytoskeleton in normal and pathological cell motility. Int. J. Biochem. Cell Biol. 2004; 36:1890-909.
- Gourlay CW and Ayscough KR. The actin cytoskeleton: a key regulator of apoptosis and ageing. Nat. Rev. 2005; 6:583-9.]