BTK Monoclonal Antibody

BTK Monoclonal Antibody | catalog# 32-123

Background

Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases.All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini.BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation.

Description

Western blot analysis using BTK monoclonal antibody against truncated BTK recombinant protein and K562 cell lysate.

Immunofluorescense analysis of Jurkat cells using BTK monoclonal antibody with FITC - IgG (top). Immunohistochemical analysis of paraffin - embedded human lymph follicle tissue showing cytoplasmic and membrane location using BTK antibody with DAB (bottom).

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Specifications

Additional Names

BTK

Clonality/Clone

This is a monoclonal antibody. Clone 7F12H4.

Isotype

IgG1

Formulation

Ascitic fluid containing 0.03% sodium azide.

Purified antibody available at no extra cost.

Host

BTK monoclonal antibody was raised in mouse.

Please use anti-mouse secondary antibodies.

Immunogen

Ni-NTA purified truncated recombinant BTK expressed in E. Coli strain BL21 (DE3).

Application

Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.

Tested Application

E, WB, IHC

Storage

BTK monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.