Additional NamesAbrineurin, Brain Derived Neurotrophic Factor, MGC34632, Neurotrophin
Clonality/CloneThis is a polyclonal antibody.
PreparationLyophilized from sterile-filtered PBS solution.
SourceProduced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBDNF (human BDNF).
PurificationAnti-hBDNF specific antibody was purified by affinity chromatography and then biotinylated.
HostBDNF antibody was raised in rabbit.
Please use rabbit secondary antibodies.
To detect hBDNF by direct ELISA (using 100 μl/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hBDNF.
To detect hBDNF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBDNF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
StorageBDNF lyophilized antibody is stable for at least 6 months when kept at -20˚C. The biotinylated antibody should be reconstituted to a concentration of 0.1 - 1.0 mg/ml with sterile PBS solution containing 0.1% BSA. This solution can be stored at 4˚C for at least one month without detectable loss of activity. Frozen aliquots of this solution are stable for at least 6 months when kept at -20˚C. Avoid more than one freeze-thaw cycle.