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BDNF Antibody | catalog# XP-5108

Background

BDNF Antibody

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Specifications

Additional Names
Abrineurin, Brain Derived Neurotrophic Factor, MGC34632, Neurotrophin
Preparation
A sterile filtered antibody solution (1 mg/mL) was lyophilized from 0.5X PBS pH 7.2.
Clonality/Clone
This is a polyclonal antibody.
Source
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBDNF (human Brain-Derived Neurotrophic Factor).
Purification
Anti-hBDNF specific antibody was purified by affinity chromatography employing immobilized hBDNF matrix.
Host
BDNF antibody was raised in rabbit.

Please use rabbit secondary antibodies.
Application
ELISA:

To detect hBDNF by direct ELISA (using 100 μl/well antibody solution) a concentration of at least 0.5μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hBDNF.

Western Blot:

To detect hBDNF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBDNF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Tested Application
E, WB
Storage
BDNF lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/ml. The antibody is stable for at least six weeks at 2-4˚C when reconstituted.
Species Reactivity
H