Additional NamesAbrineurin, Brain Derived Neurotrophic Factor, MGC34632, Neurotrophin
PreparationA sterile filtered antibody solution (1 mg/mL) was lyophilized from 0.5X PBS pH 7.2.
Clonality/CloneThis is a polyclonal antibody.
SourceProduced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBDNF (human Brain-Derived Neurotrophic Factor).
PurificationAnti-hBDNF specific antibody was purified by affinity chromatography employing immobilized hBDNF matrix.
HostBDNF antibody was raised in rabbit.
Please use rabbit secondary antibodies.
To detect hBDNF by direct ELISA (using 100 μl/well antibody solution) a concentration of at least 0.5μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hBDNF.
To detect hBDNF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBDNF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
StorageBDNF lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/ml. The antibody is stable for at least six weeks at 2-4˚C when reconstituted.