BAFF and Receptor Detection Set

BAFF and Receptor Detection Set | catalog# PSI-1803

Background

Members in the TNF superfamily regulate immune responses and induce apoptosis. Two novel members were recently identified by several groups and designated BAFF and APRIL. Both proteins stimulate B cell proliferation, humoral immune responses, NF-κB activation, and apoptosis. Two receptors, TACI and BCMA, recognize both BAFF and APRIL; a third receptor, BAFF-R, is specific for BAFF. These ligands and their receptors are involved in the development of autoimmune diseases as well as cancer.For images please see PDF data sheet

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Specifications

Buffer

Antibodies are supplied as affinity chromatography purified IgG, in PBS containing 0.02% sodium azide.

Disclaimer

This product is for research use only.

Source

Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.

Application

These polyclonal antibodies can be used for detection of BAFF and its receptors by Western blot at 2 μg/mL to 1 μg/mL and Immunohistochemistry. Whole cell lysate from HepG2 cells can be used as a positive control for each antibody except BAFF-R antibody; murine spleen lysate can be used as a positive control for that.

Tested Application

WB, IHC

Blocking Peptide

BAFF Peptide, Cat. No. 2221P

APRIL Peptide, Cat. No. 2415P

TACI Peptide, Cat. No. 2395P

BCMA Peptide, Cat. No. 2397P

BAFF-R Peptide, Cat. No. 3097P

Storage

Store antibodies at -20˚C, stable for one year.

Positive Control

HepG2 Lysate, Cat. No. 1211
Murine Spleen Lysate, Cat. No. 1406.]

Species Reactivity

see datasheet

Kit Contents

BAFF Antibody, Cat. No. 2221 (50 μg)

APRIL Antibody, Cat. No. 2415 (50 μg)

TACI Antibody, Cat. No. 2395 (50 μg)

BCMA Antibody, Cat. No. 2397 (50 μg)

BAFF-R Antibody, Cat. No. 3097 (50 μg)

Reference

Moore PA, Belvedere O, Orr A, et al. BLyS: member of the tumor necrosis factor family and B lymphocyte stimulator. >Science 1999; 285:260-3.
Schneider P, MacKay F, Steiner V, et al. BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth. J Exp Med 1999; 189:1747-56.
Shu HB, Hu WH, Johnson H. TALL-1 is a novel member of the TNF family that is down-regulated by mitogens. J Leuk Biol 1999; 65:680-3.
Hahne M, Kataoka T, Schroter M, et al. APRIL, a new ligand of the tumor necrosis factor family, stimulates tumor cell growth. J Exp Med. 1998; 188(6):1185-90.]