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B-raf Antibody | catalog# 5777

Background

B-raf belongs to the raf/mil family of serine/threonine protein kinases and plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. The Ras/Raf/MEK/ERK and Ras/PI3K/PTEN/Akt pathways interact with each other to regulate growth and in some cases tumorigenesis. Mutations in B-raf have been associated with several cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung, leading to speculation on the possibility of B-raf as a therapeutic target for treating cancers. Mutations in this gene have also been associated with cardiofaciocutaneous syndrome (CFCS), a disease characterized by heart defects, mental retardation and a distinctive facial appearance.

Description
B-raf Antibody
Western blot analysis of B-raf in human brain tissue lysate with B-raf antibody at (A) 1 and (B) 2 μg/ml.
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Specifications

Additional Names
v-raf murine sarcoma viral oncogene homolog B1, proto-oncogene B-raf, p94
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid

Concentration: 1 mg/mL
Immunogen
B-raf antibody was raised against an 18 amino acid peptide near the center of human B-raf.
Clonality/Clone
This is a polyclonal antibody.
Purification
Affinity chromatography purified via peptide column
Host
B-raf antibody was raised in chicken.

Please use anti-chicken secondary antibodies.
Application
B-raf antibody can be used for detection of B-raf by Western blot at 1 - 2 μg/ml.
Tested Application
E, WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5777P - B-raf Peptide
Storage
B-raf antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
  • Cat. No. 1303 - Human Brain Tissue Lysate]
Species Reactivity
H, M, R
Protein GI Number
50403720
Protein Accession Number
P15056
Reference
  1. McCubrey JA, Steelman LS, Chappell WH, et al. Roles of the RAF/MEK/ERK pathway in cell growth, malignant transformation and drug resistance. Biochim. Biophys. Acta2007; 1773:1263-84.
  2. Madhunapantula SV and Robertson GP. Is B-raf a good therapeutic target for melanoma and other malignancies? Cancer Res.2008; 68:5-8.
  3. Sarkozy A, Carta C, Moretti S, et al. Germline BRAF mutations in Noonan, LEOPARD, and cardiofaciocutaneous syndromes: molecular diversity and associated phenotypic spectrum. Hum. Mutat.2009; 30:695-702.]