PreparationLyophilized from sterile-filtered PBS solution.
Clonality/CloneThis is a polyclonal antibody.
SourceProduced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hβ-NGF (human β-NGF).
PurificationAnti-hβ-NGF specific antibody was purified by affinity chromatography and then biotinylated.
HostB-NGF antibody was raised in rabbit.
Please use rabbit secondary antibodies.
To detect hβ-NGF by direct ELISA (using 100 μl/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hβ-NGF.
To detect hβ-NGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hβ-NGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Storageβ-NGF lyophilized antibody is stable for at least 6 months when kept at -20˚C. The biotinylated antibody should be reconstituted to a concentration of 0.1 - 1.0 mg/ml with sterile PBS solution containing 0.1% BSA. This solution can be stored at 4˚C for at least one month without detectable loss of activity. Frozen aliquots of this solution are stable for at least 6 months when kept at -20˚C. Avoid more than one freeze-thaw cycle.