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Secondary Antibodies
Rabbit Anti-IgY Antibody, Biotin Conjugate
Background
Rabbit Anti-IgY Antibody, Biotin Conjugate
Conjugate
Biotin
Purification
Affinity
Host
Rabbit
Immunogen
Chicken Total IgY
Preparation
Rabbits were immunized with affinity-purified chicken total IgY isolated from normal chicken yolk. The resulting anti-IgY antibody was immunoaffinity purified off a chicken IgY immunoabsorbant column and conjugated with Biotin.
Application
The affinity-purified antibodies react only with chicken IgY and not IgM or IgA immunoglobulins by immunodiffusion and IEP techniques. In a sandwich ELISA against 1 µg / ml chicken IgY, conjugated antibody diluted to 0.1µg / ml produced an O.D. = 1.0 in a 15 minute reaction with TMB substrate. Suggested tissue-staining dilution: 1: 300 to 500. Optimal conjugate working dilutions should be determined for your particular assay conditions.
Tested Application
WB,E
Buffer
PBS
Storage
4˚C, avoid frequent freeze-thaw
Species Reactivity
anti-Chicken
Formulation
PBS (pH 7.4)
PRESERVATIVE:
0.1% Sodium Azide
This product belongs to the following categories:
Datasheet 06-01W
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