ANTIBODY PURIFICATION

ProSci can purify antibody from serum, ascites fluid, culture medium, or yolks using a variety of purification procedures. These procedures include protein A or G, immuno-affinity, or IgY purification.

Protein A or G:

Isolates total mammalian IgG from serum, ascites fluid, or cell culture medium. Which type is used depends on the sample's species and antibody isotype. Protein A or G is not recommended for peptide antigens as antibodies against the large carrier protein will also be isolated. This method is best used with antiserum produced using protein antigens larger than 10kDa and for purifying monoclonal antibodies from ascites or supernatant.

Immuno-Affinity:

Isolates antigen-specific antibodies using a column constructed by coupling an antigen to a gel matrix. This method is best for isolating peptide-specific antibodies from any host species. Can also be used for protein antigens and monoclonal antibodies.

IgY Purification:

Isolates total IgY from yolks. Chicken IgY is the functional equivalent of mammalian IgG but is primarily isolated from egg yolks rather than serum. This is done by removing the lipids and precipitating them from the resulting solution. The total IgY can then be used "as-is" or further purified using an immuno-affinity column.

Before and after image of crude antiserum against a peptide antigen and immuno-affinity purified antibody using the peptide.

If you have any questions regarding our antibody purification services, please contact us at abservices@prosci-inc.com, or by calling toll-free 888-513-9525.