Alkaline Phosphatase Conjugated Affinity Purified anti-Mouse IgG [H&L] [Goat] Minimum Cross reactivity to Human Serum Proteins

Alkaline Phosphatase Conjugated Affinity Purified anti-Mouse IgG [H&L] [Goat] Minimum Cross reactivity to Human Serum Proteins | catalog# XR-9780

Background

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Specifications

User Note

USDA Certification: Products of animal origin are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation.

During shipment, small volumes of Alkaline Phosphatase Conjugated Affinity Purified anti-Mouse IgG will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.

Conjugate

Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)

Purification

Alkaline Phosphatase Conjugated Affinity Purified anti-Mouse IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Human Serum Proteins.

Host

Goat

Immunogen

Mouse IgG whole molecule

Preparation

PHYSICAL STATE: Liquid (sterile filtered)

ANTIBODY CONCENTRATION: 1.0 mg/ml (by UV absorbance at 280 nm)

Application

Alkaline Phosphatase Conjugated Affinity Purified anti-Mouse IgG is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended dilutions are as follows, ELISA 1:2,000 - 1:8,000, Western Blot 1:500 - 1:4,000, Immunohistochemistry 1:500 - 1:2,000. (Optimal dilution should be determined by user).

Tested Application

E, WB, IHC

Buffer

0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0

Storage

Alkaline Phosphatase Conjugated Affinity Purified anti-Mouse IgG can be stored vial at 4˚C before opening. DO NOT FREEZE. This product is stable at 4˚C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.

Species Reactivity

Formulation

STABILIZER:10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free

PRESERVATIVE: 0.01% (w/v) Sodium Azide

Reference

Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971. ]