Akirin1 Antibody | catalog# 4799
Background
Akirin1 Antibody: The highly conserved, nuclear-localized Akirin1 and Akirin2 proteins critically regulate the transcription of NF-κB-dependent genes and are required for defense against Gram-negative bacteria in the immune deficiency and NF-κB pathways. Akirin1 is dispensable in the mouse, and neither knockout mice nor cells derived from them have obvious distinctive phenotypes. In contrast, Akirin2 is required for development in the mouse and knockout of both Akirin homologs in mice show that Akirin2 is required downstream of toll-like receptor (TLR), TNF-α and IL-1β signaling, and for the production of IL-6. Akirin2 is functionally closer to the single gene in Drosophila, as the homozygous null D. melanogaster Akirin mutants show a similar, mid-to-early embryonic death.
Description
Specifications
Additional Names
Akirin1 Antibody: Chromosome 1 Open Reading Frame 108, C1orf108, STRF2Disclaimer
This product is for research use only.User Note
Optimal dilutions for each application to be determined by the researcher.Immunogen
Akirin1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of the human Akirin1.Isotype
IgGClonality/Clone
This is a polyclonal Akirin1 Antibody.Conjugate
NoneFormulation
Physical State: LiquidConcentration: 1 mg/mL
Purification
Akirin1 Antibody is affinity chromatography purified via peptide column.Host
Akirin1 antibody was raised in rabbit.Please use anti-rabbit secondary antibodies.
Application
Akirin1 antibody can be used for detection of Akirin1 by Western blot at 1 - 2 μg/mL.Tested Application
E, WBBuffer
Akirin1 Antibody is supplied in PBS containing 0.02% sodium azide.Blocking Peptide
Cat.No. 4799P - Akirin1 PeptideStorage
Akirin1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.Positive Control
- Cat. No. 1203 - A549 Cell Lysate ]
Species Reactivity
H, M, RProtein GI Number
55962136Protein Accession Number
CAI16710Reference
- Goto A, Matsushita K, Gesellchen V, et al. Akirins are highly conserved nuclear proteins required for NF-kappaB-dependent gene expression in drosophila and mice. Nat. Immunol.2008; 9:97-104.
- Beutler B and Moresco EM. Akirins versus infection. Nat. Immunol.2008; 9:7-9.
- Sutterwala FS and Flavell RA. Immunology: cascade into clarity. Nature2008; 451:254-5.
- Tanji T and Ip YT. Regulators of the Toll and Imd pathways in the Drosophila innate immune response. Trends Immunol.2005; 26:193-8.]