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Adipose Lysate | catalog# 21-266

Background

Monkey (Rhesus) adipose tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70˚C. The monkey (Rhesus) adipose tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the adipose tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The adipose tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

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Specifications

Tissue Type
Adipose
Diagnosis
Normal
Source
Monkey (Rhesus) adipose tissue
Application
Adipose lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.
Tested Application
WB
Storage
Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -70˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
Species Reactivity
n/a
Species
Monkey (Rhesus)