Adipokines: Adiponectin and CTRPs 1-7 Detection Set

Adipokines: Adiponectin and CTRPs 1-7 Detection Set | catalog# PSI-1804

Background

Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-Α-related proteins (CTRPs) 1-7. Unlike the CTRPs, which are expressed in a wide variety of tissues, adiponectin is reported to be expressed exclusively by differentiated adipocytes. These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. Adiponectin is present in high levels in normal human plasma, but is reduced in obese subjects and often in those with increased insulin resistance and type 2 diabetes, suggesting that adiponectin may be a useful pharmacological target in various metabolic diseases. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene.For images please see PDF data sheet

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Specifications

Buffer

Antibodies are supplied as affinity chromatography purified IgG, in PBS containing 0.02% sodium azide.

Disclaimer

This product is for research use only.

Source

Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.

Application

These polyclonal antibodies can be used for detection of the indicated adipokines by Western blot at 1 μg/mL to 2 μg/mL and Immunohistochemistry.

Tested Application

WB, IHC

Blocking Peptide

Adiponectin Peptide, Cat. No. 3551P

CTRP1 Peptide, Cat. No. 3557P

CTRP2 Peptide, Cat. No. 3561P

CTRP3 Peptide, Cat. No. 3565P

CTRP4 Peptide, Cat. No. 3567P

CTRP5 Peptide, Cat. No. 3571P

CTRP6 Peptide, Cat. No. 3575P

CTRP7 Peptide, Cat. No. 3579P

Storage

Store antibodies at -20˚C, stable for one year.

Positive Control

293 Lysate (for Adiponectin and CTRP7), Cat. No. 1210

Human Kidney Lysate (for CTRP1), Cat. No. 1305

Caco-2 Lysate (for CTRP2), Cat. No. 1223

Mouse Heart Lysate (for CTRP3), Cat. No. 1401

Rat Brain Lysate (for CTRP4), Cat. No. 1463

Human Brain Lysate (for CTRP5), Cat. No. 1303

HeLa Lysate (for CTRP6), Cat. No. 1201]

Species Reactivity

see datasheet

Kit Contents

Adiponectin Antibody (CT), Cat. No. 3551 (50 μg)

CTRP1 Antibody (NT), Cat. No. 3557 (50 μg)

CTRP2 Antibody (NT), Cat. No. 3561 (50 μg)

CTRP3 Antibody (IN), Cat. No. 3565 (50 μg)

CTRP4 Antibody (CT), Cat. No. 3567 (50 μg)

CTRP5 Antibody (NT), Cat. No. 3571 (50 μg)

CTRP6 Antibody (CT), Cat. No. 3575 (50 μg)

CTRP7 Antibody (CT), Cat. No. 3579 (50 μg)

Reference

Fantuzzi G. Adipose tissue, adipokines, and inflammation. J. Allergy Clin. Immunol. 2005; 115:911-9.
Tsao T-S, Lodish HF, and Fruebis J. ACRP30, a new hormone controlling fat and glucose metabolism. Euro. J. Pharmacol. 2002; 440:213-21.
Wong GW, Wang J, Hug C, et al. A family of Acrp30/ adiponectin structural and functional paralogs. Proc. Natl. Acad. Sci. USA 2004; 101:10302-7.
Lihn AS, Pedersen SB, and Richelsen B. Adiponectin: action, regulation and association to insulin sensitivity. Obes. Rev. 2005; 6:13-21.]