Acrp30 Recombinant Protein | catalog# 40-327
Background
gAcrp30 is a naturally occurring globular protein, obtained by proteolytic processing of adiponectin. Adiponectin is produced and secreted exclusively by adipocytes, and is a relatively abundant plasma protein, accounting for up to 0.05% of total serum protein. Like Adiponectin, gAcrp30 is capable of decreasing hyperglycemia and reversing insulin resistance. Additionally, gAcrp30 has been shown to be an important factor in promoting fat loss by signaling muscle to absorb and burn Free-Fatty Acids (FFAs). The signaling receptors for adiponectin and gAcrp30 have recently been identified and names AdipoR1 and AdipoR2. AdipoR2 is predominantly expressed in the liver. Recombinant human gAcrp30/Adipolean is a 16.6 kDa protein consisting of 145 amino acid residues.
Specifications
Additional Names
apm-1, Adipolean (human)Preparation
Biological activity was determined by its ability to inhibit the proliferation of murine M1 cells. The expected ED50 for this effect is 1.0 - 3.0 ?g/ml.Location
MKGEPGEGAY VYRSAFSVGL ETYVTIPNMP IRFTKIFYNQ QNHYDGSTGK FHCNIPGLYY FAYHITVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTNSource
E. coliReactivity
n/aTested Application
n/aStorage
The lyophilized Acrp30 recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted Acrp30 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing Acrp30 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.Species Reactivity
n/aPurity
Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).