Additional NamesA-PROLIFERATION-INDUCING LIGAND, TNFSF 13
Clonality/CloneThis is a monoclonal antibody.
SourceAPRIL (monoclonal Sacha-2) antibody was raised against recombinant human APRIL (aa. 105-250).
PurificationAPRIL (monoclonal Sacha-2) antibody was purified from concentrated hybridoma tissue culture supernatant. APRIL (monoclonal Sacha-2) antibody was purified to 95% in SDS-PAGE and is supplied at a concentration of 1 mg/1ml.
HostAPRIL antibody was raised in rat.
Please use anti-rat IgG secondary antibodies
ImmunogenThe immunogen is recombinant human APRIL, amino acids 105-250, fused to human ACRP30 headless, amino acids 16-108.
ApplicationAPRIL antibody can be used to recognize human and mouse APRIL by ELISA (capture), Flow Cytometry, and Immunocytochemistry. Method: HEK-293T cells expressing surface membrane associated human APRIL (aa 92-233) or a mock control were injected in chicken liver and frozen in OCT. 8µm sections of injected frozen liver were fixed in acetone, washed in PBS and incubated with Sacha-2 at 10µg/ml for 1hr after blocking in PBS/1%BSA. Human APRIL was revealed with 3-amino-9-Ethyl-carbazole (AEC) following 45 min. incubation with goat anti-rat HRP. Method: HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of mouse APRIL (thick line). Cells were incubated on ice for 30 min in 50µl FACS buffer (PBS, 5% Fetal calf serum, 0.02% sodium azide) containing 1μg/ml of Sacha-2 antibody. After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry. This product is for research use only.
BufferAPRIL antibody is supplied as a liquid in PBS containing 0.02% sodium azide.
StorageAPRIL antibody can be stored at 4˚C, stable for one year.