Additional NamesA-PROLIFERATION-INDUCING LIGAND, TNFSF 13
Clonality/CloneThis is a monoclonal antibody.
SourceAPRIL (monoclonal Sache-1) antibody was raised against recombinant human APRIL (aa. 105-250).
PurificationAPRIL (monoclonal Sache-1) antibody was purified to ≥95% in SDS-PAGE and is supplied at a concentration of 1mg/1ml.
HostAPRIL antibody was raised in rat.
Please use anti-rat IgG secondary antibodies
ImmunogenThe immunogen is recombinant human APRIL (aa. 105-250) fused to human ACRP30headless (aa. 16-108).
ApplicationAPRIL antibody can be used to recognize human and mouse APRIL by Flow Cytometry (excellent for mouse APRIL, also stains human APRIL), and Immunocytochemistry. Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with acetone. Slides were blocked with IgG, and incubated for 1 hour with 5µg/ml Sacha-1 or control murine IgG (isotype control) in 1%BSA / 1x PBS for 1 hour. After washes wish PBS, samples were incubated with the secondary Ab for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC. Method: 3 HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of mAPRIL (thick line). Cells were incubated on ice for 30 min in 50µl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1μg/ml of Sacha-1 antibody. After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.
BufferAPRIL antibody is supplied as a liquid in PBS containing 0.02% sodium azide.
StorageAPRIL antibody can be stored at 4˚C, stable for one year.