Additional NamesA-PROLIFERATION-INDUCING LIGAND, TNFSF 13
Clonality/CloneThis is a monoclonal antibody.
SourceAPRIL (monoclonal Aprily-1) antibody was raised against recombinant human APRIL (aa. 93-233).
PurificationAPRIL (monoclonal Aprily-1) antibody was purified to >=95% in SDS-PAGE and is supplied at a concentration of 1 mg/1ml.
HostAPRIL antibody was raised in mouse.
Please use anti-mouse IgG secondary antibodies
ImmunogenThe immunogen is recombinant human APRIL, amino acids 93-233, expressed in bacteria.
ApplicationAPRIL antibody can be used to recognize human APRIL by Flow Cytometry (also works for intracellular FC staining) and Western Blot. Anti-APRIL (MAb Aprily-1) does not cross react with mouse APRIL. Method: HEK 293 cells were mock transfected or transfected with an expression plasmid enabling surface expression of hAPRIL. Cells (5x105) were incubated on ice for 30 min in 50 µl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 10µg/ml of Aprily-1 antibody. After washing in FACS buffer, FITC-conjugated antibody to mouse IgG was added. Cells were incubated on ice for 30 min, washed and analyzed by flow cytometry. Method: Cell supernatants were first precleared with protein-A sepharose and then subjected to immunoprecipitation with rhBCMA:Fc and protein-A sepharose. Beads were washed twice with PBS. Immunoprecipitated materials were eluted with 2.5mM glycine pH 2.5 for 10 min, neutralized with Tris buffer and run on an SDS-PAGE gel and analyzed with 2µg/ml Aprily-1 by Western blot. Note: In some migrations endogenous soluble APRIL resolved as a doublet around 20kDa (dendritic cells: DC). rhsMegaAPRIL served as a positive control. Method: 3 HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of mDR6 (thick line). Cells were incubated on ice for 30 min in 50µl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1μg/ml of Luke-1 antibody. After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.
BufferAPRIL antibody is supplied as a liquid in PBS containing 0.02% sodium azide.
StorageAPRIL antibody can be stored at 4˚C, stable for one year.
- BAFF production by antigen-presenting cells provides T cell co-stimulation: Int. Immunol.; B. Huard, et al. 16, 467 (2004).
- BAFF and APRIL: A Tutorial on B Cell Survival: F. Mackay, et al.; Annu. Rev. Immunol. (2002).]