APRIL Antibody | catalog# XP-5103Bt
Background
APRIL Biotin Conjugated Antibody
Specifications
Additional Names
A proliferation inducing ligand, CD256, Proliferation inducing ligand APRIL, TALL2, TNF and APOL related leukocyte expressed ligand 2, TNF related death ligand, TNFSF13, TRDL1, Tumor necrosis factor (ligand) superfamily member 13, Tumor necrosis factor related death ligand, TWE PRIL, ZTNF2Preparation
Lyophilized from sterile-filtered PBS solution.Clonality/Clone
This is a polyclonal antibody.Conjugate
BiotinSource
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hAPRIL (human APRIL).Host
APRIL antibody was raised in rabbit.Please use rabbit secondary antibodies.
Application
ELISA:To detect hAPRIL by direct ELISA (using 100 μl/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hAPRIL.
Western Blot:
To detect hAPRIL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hAPRIL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Tested Application
E, WBStorage
APRIL lyophilized antibody is stable for at least 6 months when kept at -20˚C. The biotinylated antibody should be reconstituted to a concentration of 0.1 - 1.0 mg/ml with sterile PBS solution containing 0.1% BSA. This solution can be stored at 4˚C for at least one month without detectable loss of activity. Frozen aliquots of this solution are stable for at least 6 months when kept at -20˚C. Avoid more than one freeze-thaw cycle.Positive Control
- Cat. No. 1204 - K562 Cell Lysate]