APRIL Antibody | catalog# XP-5103
Background
APRIL Antibody
Specifications
Additional Names
A proliferation inducing ligand, CD256, Proliferation inducing ligand APRIL, TALL2, TNF and APOL related leukocyte expressed ligand 2, TNF related death ligand, TNFSF13, TRDL1, Tumor necrosis factor (ligand) superfamily member 13, Tumor necrosis factor related death ligand, TWE PRIL, ZTNF2Clonality/Clone
This is a polyclonal antibody.Preparation
A sterile filtered antibody solution (1 mg/mL) was lyophilized from 0.5X PBS pH 7.2.Source
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hAPRIL (human APRIL).Host
APRIL antibody was raised in rabbit.Please use rabbit secondary antibodies.
Application
ELISA:To detect hAPRIL by direct ELISA (using 100 μl/well antibody solution) a concentration of at least 0.5μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hAPRIL.
Western Blot:
To detect hAPRIL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hAPRIL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Tested Application
E, WBStorage
APRIL lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/ml. The antibody is stable for at least six weeks at 2-4˚C when reconstituted.Positive Control
- Cat. No. 1204 - K562 Cell Lysate]