APRIL Antibody | catalog# 2223
Background
APRIL Antibody: Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated APRIL (for a proliferation-inducing ligand), TALL-2 (for TNF- and ApoL-related Leukocyte-expressed Ligand 2), and TRDL-1α (for TNF related death ligand 1α) in human and mouse. Two receptors for APRIL were recently identified and designated TACI and BCMA. APRIL stimulates B and T cell proliferation, triggers humoral immune responses, activates NF-κB, and induces cell death. APRIL and its close relative of BlyS and their receptors BCMA and TACI are involved in diseases of autoimmunity and cancer.
Description
Western blot analysis of APRIL expression in K562 cells with APRIL antibody at (A) 5 and (B) 10 μg/mL.
Specifications
Additional Names
APRIL (ED), APRILAdditional Names
APRIL Antibody:User Note
Optimal dilutions for each application to be determined by the researcher.Isotype
IgGMolecular Weight
27 kDaDisclaimer
This product is for research use only.Formulation
Physical State: LiquidConcentration: 1 mg/mL
Conjugate
NoneClonality/Clone
This is a polyclonal APRIL Antibody.Purification
APRIL Antibody is affinity chromatography purified via peptide column.Host
APRIL antibody was raised in rabbit.Please use anti-rabbit secondary antibodies.
Immunogen
APRIL antibody was raised against a synthetic peptide corresponding to amino acids near the center of human APRIL.Application
APRIL can be used for detection of APRIL by Western blot at 5 - 10 μg/mL. A 27 kDa band can be detected.Tested Application
E, WBBuffer
APRIL Antibody is supplied in PBS containing 0.02% sodium azide.Blocking Peptide
Cat. No. 2223P - APRIL PeptideStorage
APRIL antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.Positive Control
- Cat. No. 1204 - K562 Cell Lysate]
Species Reactivity
HProtein GI Number
4507599Protein Accession Number
NP_003799Reference
- Hahne M, Kataoka T, Schroter M, et al. APRIL, a new ligand of the tumor necrosis factor family, stimulates tumor cell growth. J Exp Med. 1998;188(6):1185-90.
- Shu HB, Hu WH, Johnson H. TALL-1 is a novel member of the TNF family that is down-regulated by mitogens. J Leukoc Biol. 1999;65(5):680-3.
- Kelly K, Manos E, Jensen G, et al. APRIL/TRDL-1, a tumor necrosis factor-like ligand, stimulates cell death. Cancer Res. 2000;60(4):1021-7.
- Yu G, Boone T, Delaney J, et al. APRIL and TALL-I and receptors BCMA and TACI: system for regulating humoral immunity. Nat Immunol. 2000;1(3):252-6. ]