APOBEC3G Antibody

APOBEC3G Antibody | catalog# 27-882

Background

APOBEC3G is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. The protein encoded by this gene has been found to be a specific inhibitor of human immunodeficiency virus-1 (HIV-1) infectivity.

Description

Western blot analysis of APOBEC3G in HepG2 Cell Lysate using APOBEC3G antibody at 2 μg/ml (12% gel).

Credit Card Processing

Specifications

Additional Names

APOBEC3G

Disclaimer

This product is for research use only.

Positive Control

Cat. No. 1211 - HepG2 Cell Lysate]

Clonality/Clone

This is a polyclonal antibody.

Purification

Antibody is purified by peptide affinity chromatography method.

Host

APOBEC3G antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.

Immunogen

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human APOBEC3G.

Application

APOBEC3G antibody can be used for detection of APOBEC3G by ELISA at 1:1562500. APOBEC3G antibody can be used for detection of APOBEC3G by western blot at 2.0 μg/ml, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Tested Application

E, WB, IHC

Buffer

Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml.

Storage

For short periods of storage (days) store at 4˚C. For longer periods of storage, store APOBEC3G antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.