ANXA1 Antibody | catalog# 28-512
Background
ANXA1 encodes a protein that belongs to a family of Ca (2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity.
Description
Western blot analysis of ANXA1 in HepG2 Cell Lysate using ANXA1 antibody at 1.25 μg/ml (12% gel).
Specifications
Additional Names
ANXA1Positive Control
- Cat. No. 1211 - HepG2 Cell Lysate]
Disclaimer
This product is for research use only.Clonality/Clone
This is a polyclonal antibody.Purification
Antibody is purified by protein A chromatography method.Host
ANXA1 antibody was raised in rabbit.Please use anti-rabbit secondary antibodies.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ANXA1.Application
ANXA1 antibody can be used for detection of ANXA1 by ELISA at 1:62500. ANXA1 antibody can be used for detection of ANXA1 by western blot at 1.25 μg/ml, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.Tested Application
E, WB, IHCBuffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μl of distilled water. Final antibody concentration is 1 mg/ml.Storage
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ANXA1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.Species Reactivity
HProtein Accession Number
NP_000691Molecular Weight
38 kDaReference
- Ernst, S., et al., (2004) J. Immunol. 172 (12), 7669-7676.]