ANP32A Antibody | catalog# 29-209
Background
ANP32A is a novel potent heat-stable inhibitor protein of protein phosphatase 2A. It may play a key role in self-renewing cell populations where it may act in the nucleus to limit their sensitivity to transformation. Being a tumor suppressor, ANP32A represses cell growth through inhibition of transcription by blocking acetylation and phosphorylation of histone H3 and initiating its proapoptotic activity.
Description
Western blot analysis of ANP32A in Jurkat Cell Lysate using ANP32A antibody at 0.25 μg/ml (12% gel). Marker in lane A.
Specifications
Additional Names
ANP32APositive Control
- Cat. No. 1205 - Jurkat Cell Lysate]
Disclaimer
This product is for research use only.Clonality/Clone
This is a polyclonal antibody.Purification
Antibody is purified by peptide affinity chromatography method.Host
ANP32A antibody was raised in rabbit.Please use anti-rabbit secondary antibodies.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ANP32A.Application
ANP32A antibody can be used for detection of ANP32A by ELISA at 1:312500. ANP32A antibody can be used for detection of ANP32A by western blot at 0.25 μg/ml, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.Tested Application
E, WB, IHCBuffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml.Storage
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ANP32A antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.Species Reactivity
HProtein Accession Number
NP_006296Molecular Weight
29 kDaReference
- Stelzl, U., (2005) Cell 122 (6), 957-968.]