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AK2 Antibody | catalog# 30-145

Background

Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis.Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Two transcript variants encoding distinct isoforms have been identified for this gene.

Description
AK2 Antibody
Western blot analysis of AK2 in HepG2 Cell Lysate using AK2 antibody at 2.5 μg/ml (12% gel).
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Specifications

Additional Names
AK2, ADK2
Positive Control
  • Cat. No. 1211 - HepG2 Cell Lysate]
Disclaimer
This product is for research use only.
Clonality/Clone
This is a polyclonal antibody.
Purification
Antibody is purified by protein A chromatography method.
Host
AK2 antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AK2.
Application
AK2 antibody can be used for detection of AK2 by ELISA at 1:62500. AK2 antibody can be used for detection of AK2 by western blot at 2.5 μg/ml, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Tested Application
E, WB
Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μl of distilled water. Final antibody concentration is 1 mg/ml.
Storage
For short periods of storage (days) store at 4˚C. For longer periods of storage, store AK2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Species Reactivity
H
Protein Accession Number
NP_001616
Molecular Weight
26 kDa, 26 kDa
Reference
  1. Kohler, C., (1999) FEBS Lett. 447 (1), 10-12.]