AIRE Antibody | catalog# 27-660
Background
AIRE is a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CBP. At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD). Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).
Description
Western blot analysis of AIRE in HepG2 Cell Lysate using AIRE antibody at 5 μg/ml (12% gel). Marker in lane A.
Specifications
Additional Names
AIREPositive Control
- Cat. No. 1211 - HepG2 Cell Lysate]
Disclaimer
This product is for research use only.Clonality/Clone
This is a polyclonal antibody.Purification
Antibody is purified by protein A chromatography method.Host
AIRE antibody was raised in rabbit.Please use anti-rabbit secondary antibodies.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AIRE.Application
AIRE antibody can be used for detection of AIRE by ELISA at 1:1562500. AIRE antibody can be used for detection of AIRE by western blot at 5 μg/ml, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.Tested Application
E, WBBuffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μl of distilled water. Final antibody concentration is 1 mg/ml.Storage
For short periods of storage (days) store at 4˚C. For longer periods of storage, store AIRE antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.Species Reactivity
HProtein Accession Number
NP_000374Molecular Weight
58 kDaReference
- Tao, Y., et al., (2006) Mol. Immunol. 43 (4), 335-345.]