AID Antibody | catalog# 3091
Background
AID Antibody: Activation-induced cytidine deaminase (AID) was initially discovered as a homolog of the apolipoprotein B RNA-editing cytidine deaminase 1 (APOBEC1) that showed cytidine deaminase properties in stimulated B cell lines. It is necessary for somatic hypermutation and class switch recombination in B cells, but inappropriate or dysregulated expression AID is often found in tumors and B cell neoplasms. Although it is structurally and functionally similar to the APOBEC proteins, it appears unlikely that AID deaminates dC to dU residues in HIV cDNA as does APOBEC3G.
Description
Western blot analysis of AID in Ramos whole cell lysate with AID antibody at 2 μg/mL in either the (A) absence or (B) presence of blocking peptide.
Immunocytochemistry of AID in Ramos cells with Ramos antibody at 10 μg/mL.
Specifications
Additional Names
AIDAdditional Names
AID Antibody:User Note
Optimal dilutions for each application to be determined by the researcher.Isotype
IgGDisclaimer
This product is for research use only.Formulation
Physical State: LiquidConcentration: 1 mg/mL
Conjugate
NoneClonality/Clone
This is a polyclonal AID Antibody.Purification
AID Antibody is affinity chromatography purified via peptide column.Host
AID antibody was raised in rabbit.Please use anti-rabbit secondary antibodies.
Immunogen
AID antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human AID.Application
AID antibody can be used for detection of AID by Western blot at 2 μg/mL. Anti-AID is human and mouse reactive. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Tested Application
E, WB, ICC, IFBuffer
AID Antibody is supplied in PBS containing 0.02% sodium azide.Blocking Peptide
Cat. No. 3091P - AID PeptideStorage
AID antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.Positive Control
- Cat. No. 1225 - Ramos Cell Lysate]
Species Reactivity
H, MProtein GI Number
22297218Protein Accession Number
AAM95402Reference
- Muramatsu M, Sankaranand VS, Anant S, et al. Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells. J. Biol. Chem. 1999; 274:18470-6.
- Muramatsu M, Kinoshita K, Fagarasan S, et al. Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme.Cell 2000; 102:553-63.
- Greeve J, Philipsen A, Krause K, et al. Expression of activation-induced cytidine deaminase in human B-cell Hodgkin lymphomas. Blood 2003; 101:3574-80.
- Cascalho M. Advantages and disadvantages of cytidine deamination. J. Immunol. 2004; 172:6513-8.]