AFAP1 Antibody | catalog# 6557
Background
The actin filament-associated protein AFAP1 (AFAP-110) is an actin cross-linking protein first identified as a substrate of the viral oncogene v-Src . AFAP1 has a fundamental role in actin cytoskeleton arrangement . It contains a carboxyterminal actin-binding domain that directly binds to F-actin and serves as an adaptor protein in the regulation of SRC and PKC signal transduction by several functional domains, including 2 pleckstrin homology (PH) domains, a Src homology 3–binding (SH3-binding) motif, and several SH2-binding motifs . It is overexpressed in prostate carcinoma and contributes to tumor growth by regulating cell-matrix adhesions and migration in the cancer cells. AFAP1 represent a possible therapeutic target for controlling tumorigenesis and metastasis .
Description
Specifications
Protein Accession Number
NP_940997Purification
Affinity chromatography purified via peptide columnClonality/Clone
This is a polyclonal antibody.Host
AFAP1 antibody was raised in rabbit.Please use anti-rabbit secondary antibodies.
Specificity
Monomer and homomultimer of AFAP1 are known to exist; AFAP1 antibody is predicted to not cross-react with other AFAP family members.Immunogen
AFAP1 antibody was raised against an 18 amino acid peptide near the amino terminus of human AFAP1.Application
AFAP1 antibody can be used for detection of AFAP1 by Western blot at 1 - 2 μg/mL.Tested Application
E, WBBuffer
Antibody is supplied in PBS containing 0.02% sodium azide.Blocking Peptide
Cat. No. 6557 - AFAP1 PeptideStorage
AFAP1 Antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.Reference
- Flynn DC, Leu TH, Reynolds AB, et al. Identification and sequence analysis of cDNAs encoding a 110-kilodalton actin filament-associated pp60src substrate. Mol. Cell. Biol. 1993;13:7892–900.
- Baisden JM, Gatesman AS, Cherezova L, et al. The intrinsic ability of AFAP-110 to alter actin filament integrity is linked with its ability to also activate cellular tyrosine kinases. Oncogene 2001; 20:6607–16.
- Baisden JM, Qian Y, Zot HM, et al. The actin filament-associated protein AFAP-110 is an adaptor protein that modulates changes in actin filament integrity. Oncogene 2001; 20:6435–47.
- Zhang J, Park SI, Artime MC, et al. AFAP-110 is overexpressed in prostate cancer and contributes to tumorigenic growth by regulating focal contacts. J. Clin. Invest. 2007; 117:2962-73.]
Positive Control
- Cat. No. 1201 - HeLa Cell Lysate]