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ABIN3 Antibody | catalog# 5303

Background

ABIN3 Antibody: The nuclear factor NF-κB plays key roles in development and immunity. ABIN3 (A20-binding inhibitor of NF-κB activation 3), also known as TNFAIP3-interacting protein 3 (TNIP3), is a novel negative feedback regulator of LPS-induced NF-κB activation. ABIN3 is a 39 kDa protein that negatively regulates NF-κB activation in response to TNF and LPS. ABIN3 is highly expressed in brain, thymus, lymph node, lung and fetal liver, with low expression in kidney, bone marrow. Through its interaction with A20, ABIN3 interferes with TRAF2-mediated transactivation signals and NF-κB inhibition is mediated by the ABIN-homology domain 2. ABIN3 has been found to be induced by Listeria infection and can be slightly downregulated by dexamethasone. Enhanced expression of ABIN3 in monocytes is associated with sepsis. Thus, ABIN3 is an IL-10-induced gene product capable of attenuating NF-κB in human macrophages yet is inoperative in mice and represents a basis for species-specific differences in IL-10 actions. At least four isoforms of ABIN3 are known to exist.

Description
ABIN3 Antibody
Western blot analysis of ABIN3 in human spleen tissue lysate with ABIN3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
ABIN3 Antibody
Immunohistochemistry of ABIN3 in mouse spleen tissue with ABIN3 antibody at 5 μg/mL.
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Specifications

Additional Names
ABIN3 Antibody: A20-binding inhibitor of NF-kappaB activation 3, TNFAIP3 interacting protein 3, TNIP3, Listeria-induced gene protein, LIND
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Immunogen
ABIN3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human ABIN3.
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid

Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal ABIN3 Antibody.
Purification
ABIN3 Antibody is affinity chromatography purified via peptide column.
Host
ABIN3 antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.
Application
ABIN3 antibody can be used for detection of ABIN3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Tested Application
E, WB, IHC
Buffer
ABIN3 Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5303P - ABIN3 Peptide
Storage
ABIN3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
  • Cat. No. 1306 - Human Spleen Tissue Lysate]
Species Reactivity
H, M
Protein GI Number
192807305
Protein Accession Number
NP_079149
Reference
  1. Verstrepen L, Carpentier I, Verhelst K, et al. Abins: A20 binding inhibitors of NFkappaB and apoptosis signaling. Biochem. Pharmacol.2009; 78:105-14.
  2. Bouwmeester T, Bauch A, Ruffner H, et al. A physical and functional map of the human TNFα/NFkappaB signal transduction pathway. Nat. Cell Biol.2004; 6:97-105.
  3. Wullaert A, Verstrepen L, Van Huffel S, et al. LIND/Abin-3 is a novel lipopolysaccharide-inducible inhibitor of NFkappaB activation. J. Biol. Chem.2007; 282:81-90.
  4. Weaver BK, Bohn E, Judd BA, et al. Abin-3: a molecular basis for species divergence in interleukin-10-induced anti-inflammatory actions. Mol. Cell. Biol.2007; 27:4603-16.]