ABCB5 Antibody | catalog# 46-620
Background
Description
Western blot analysis of ABCB5 in A431 cell lysate (35μg protein in RIPA buffer) using ABCB5 antibody (0.03μg/mL).
Specifications
Additional Names
ATP-binding cassette, sub-family B (MDR/TAP), member 5, ABCB5alpha, ABCB5beta, EST422562, ATP-binding cassette, sub-family B, member 5N: P-glycoprotein ABCB5Preparation
Physical State: LiquidConcentration: 500 μg/mL
Immunogen
ABCB5 antibody was raised against a 13 amino acid synthetic peptide near the internal region of ABCB5.Disclaimer
This product is for research use only.Clonality/Clone
This is a polyclonal antibody.Source
ABCB5 antibody was raised against a synthetic peptide of ABCB5.Purification
ABCB5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.Host
ABCB5 antibody was raised in goat.Please use anti-goat secondary antibodies.
Application
ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 80 kDa band observed in lysates of A431. Recommended concentration: 0.03-0.1 μg/mL. Approximately 90 kDa band observed in human skin lysates and in lysates of cell line A431 (calculated MW of 89.8 kDa according to NP_848654.3). Recommended concentration: 0.3-1 μg/mL.Tested Application
E, WB, FCBuffer
ABCB5 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.Blocking Peptide
Cat. No. 46-620P - ABCB5 PeptideStorage
Aliquot and store at -20˚C. Minimize freezing and thawing.Positive Control
- Cat. No. 1202 - A431 Cell Lysate]
Species Reactivity
HProtein Accession Number
NP_848654.3Reference
- Frank NY, Margaryan A, Huang Y, Schatton T, Waaga-Gasser AM, Gasser M, Sayegh MH, Sadee W, Frank MH. ABCB5-mediated doxorubicin transport and chemoresistance in human malignant melanoma. Cancer Res. 2005 May 15;65(10):4320-33.]