Additional NamesATP-binding cassette, sub-family B (MDR/TAP), member 5, ABCB5alpha, ABCB5beta, EST422562, ATP-binding cassette, sub-family B, member 5N: P-glycoprotein ABCB5
PreparationPhysical State: Liquid
Concentration: 500 μg/mL
ImmunogenABCB5 antibody was raised against a 13 amino acid synthetic peptide near the internal region of ABCB5.
DisclaimerThis product is for research use only.
Clonality/CloneThis is a polyclonal antibody.
SourceABCB5 antibody was raised against a synthetic peptide of ABCB5.
PurificationABCB5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
HostABCB5 antibody was raised in goat.
Please use anti-goat secondary antibodies.
ApplicationELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approximately 80 kDa band observed in lysates of A431. Recommended concentration: 0.03-0.1 μg/mL. Approximately 90 kDa band observed in human skin lysates and in lysates of cell line A431 (calculated MW of 89.8 kDa according to NP_848654.3). Recommended concentration: 0.3-1 μg/mL.
BufferABCB5 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Blocking PeptideCat. No. 46-620P - ABCB5 Peptide
StorageAliquot and store at -20˚C. Minimize freezing and thawing.
- Cat. No. 1202 - A431 Cell Lysate]
Protein Accession NumberNP_848654.3
- Frank NY, Margaryan A, Huang Y, Schatton T, Waaga-Gasser AM, Gasser M, Sayegh MH, Sadee W, Frank MH. ABCB5-mediated doxorubicin transport and chemoresistance in human malignant melanoma. Cancer Res. 2005 May 15;65(10):4320-33.]