Additional NamesABCB10, ATP-binding cassette, sub-family B (MDR/TAP), member 10, EST20237, M-ABC2, MTABC2, ATP-binding cassette, sub-family B, member 10, ABC-me
Clonality/CloneThis is a polyclonal antibody.
ReactivityTested Species Reactivity: Human
Expected species reactivity: Human, Murine, Rattus
ImmunogenABCB10 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of ABCB10.
PreparationPhysical State: Liquid
Concentration: 500 μg/mL
DisclaimerThis product is for research use only.
SourceABCB10 antibody was raised against a synthetic peptide of ABCB10.
PurificationABCB10 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
HostABCB10 antibody was raised in goat.
Please use anti-goat secondary antibodies.
ApplicationELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 90 kDa and 50 kDa in human bone marrow lysates after 0.05 μg/mL antibody staining.1 kDa according to NP_036221.2 and given the expected mature size of ~65 kDa according to Graf et al. (PMID: 15215243). Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
BufferABCB10 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Blocking PeptideCat. No. 45-179P - ABCB10 Peptide
StorageAliquot and store at -20˚C. Minimize freezing and thawing.
Protein Accession NumberNP_036221.2
- Graf SA, Haigh SE, Corson ED, Shirihai OS. Targeting, import, and dimerization of a mammalian mitochondrial ATP binding cassette (ABC) transporter, ABCB10 (ABC-me). J Biol Chem. 2004 Oct 8;279(41):42954-63]