Source
human lung carcinomaPreparation
Human A549 cells were cultured in Ham's F12K medium and harvested at the log phase of growth. The A549 cells were fixed in 4% paraformaldehyde and arrayed on a 12-well (5 mm) adhesive coated slide, with each well's surface specifically treated to enhance cellular attachment and to minimize background staining. Each well was loaded with approximately 7.5 x 103 A549 cells. A follow-up heat dehydration process was done to ensure the attachment of A549 cells and the stability of cellular proteins.Application
A549 cell slide is provided for immunocytochemical analysis of protein and protein-protein interaction. Before applying immunocytochemistry, 0.1% Triton X-100 pretreatment for 1 minute at room temperature is highly suggested.Tested Application
ICCStorage
A549 cell slide can be stored for up to 6 months at 4°C under desiccate conditions.Species Reactivity
n/aSpecies
Human
