A431 Membrane Lysate

A431 Membrane Lysate | catalog# XBL-10438

Background

A431 (Human Epidermoid Carcinoma) cell membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The A431 (Human Epidermoid Carcinoma) cell was frozen in liquid nitrogen immediately after excision and then stored at -70˚C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated A431 (Human Epidermoid Carcinoma) cell membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated A431 (Human Epidermoid Carcinoma) cell membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

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Specifications

Tissue Type

A431 (Human Epidermoid Carcinoma)

Diagnosis

Tumor

Tested Application

Source

A431 (Human Epidermoid Carcinoma) cell line

Application

A431 (Epidermoid Carcinoma) cell membrane protein lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.

Storage

Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -70˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.