Source
Fibroblast from NIH Swiss mouse embryosPreparation
Mouse NIH/3T3 cells were cultured in DMEM with 4 mM L-glutamine and harvested at the log phase of growth. The NIH/3T3 cells were fixed in 4% paraformaldehyde and arrayed on a 12-well (5 mm) adhesive coated slide, with each well's surface specifically treated to enhance cellular attachment and to minimize background staining. Each well was loaded with approximately 7.5 x 103 NIH/3T3 cells. A follow-up heat dehydration process was done to ensure the attachment of NIH/3T3 cells and the stability of cellular proteins.Application
NIH/3T3 cell slide is provided for immunocytochemical analysis of protein and protein-protein interaction. Procedures for immunohistochemistry can be directly applied.Tested Application
ICCStorage
NIH/3T3 cell slide can be stored for up to 6 months at 4°C under desiccate conditions.Species Reactivity
n/aSpecies
Mouse
