Protein Accession Number
NP_699163Location
3 prime Exonuclease peptide corresponds to 14 amino acids of 3 prime Exonuclease.Preparation
3 prime Exonuclease peptide is supplied as 100μg of dried peptide. When the peptide is reconstituted in 200μl water, the concentration will be 0.5mg/ml.Application
3 prime Exonuclease peptide is used for blocking the activity of 3 prime Exonuclease antibody (catalog number 45-176). The 3 prime Exonuclease antibody ships at 0.5mg/ml and the 3 prime Exonuclease peptide ships as a 100μl pellet. When peptide is reconstituted in 200μl water, the concentration will be 0.5mg/ml, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.Tested Application
BlBuffer
Dried peptide.Storage
Store at -20˚C. Minimize freezing and thawing.Species Reactivity
n/a
