Source
Human (embryonal) kidney transformed by sheared human adenovirus 5 (Ad 5) DNAPreparation
Human 293 cells were cultured in Minimum Essential Medium (Eagle) with 2 mM L-glutamine and harvested at the log phase of growth. The 293 cells were fixed in 4% paraformaldehyde and arrayed on a 12-well (5 mm) adhesive coated slide, with each well's surface specifically treated to enhance cellular attachment and to minimize background staining. Each well was loaded with approximately 7.5 x 103 293 cells. A follow-up heat dehydration process was done to ensure the attachment of 293 cells and the stability of cellular proteins.Application
293 cell slide is provided for immunocytochemical analysis of protein and protein-protein interaction. Procedures for immunohistochemistry can be directly applied.Tested Application
ICCStorage
293 cell slide can be stored for up to 6 months at 4°C under desiccate conditions.Species Reactivity
n/aSpecies
Human
